dapi staining solution (Beyotime)
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dapi staining solution
Dapi Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 2054 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+stain/DAPI+Staining+Solution/pmc13097096-64-0-21
Average 99 stars, based on 2054 article reviews
Dapi Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 2054 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dapi+stain/DAPI+Staining+Solution/pmc13097096-64-0-21
Average 99 stars, based on 2054 article reviews
dapi staining solution - by Bioz Stars,
2026-10
99/100 stars
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Incubation:Article Title: Biodegradable Janus sonozyme with continuous reactive oxygen species regulation for treating infected critical-sized bone defects. Article Snippet: .. The cells were covered with primary antibody of the appropriate genus and incubated at room temperature for 60min, washed with 0.1% Phosphate Buffer Saline Tween (PBST), then Article Title: Efficient, High-Quality Engineering of Therapeutic Extracellular Vesicles on an Integrated Nanoplatform. Article Snippet: Engineered extracellular vesicles (EVs) have been recognized as important therapeutics for gene and cell therapy.. To achieve clinically desired therapy, technologies for EV engineering have high demands on the efficacy in producing EVs and their qualities, which, however, remain challenging to conventional routes due to their limited control on therapeutic payload delivery, EV secretion, and extracellular microenvironments.. Here, we report a nanoplatform (denoted as PURE) that enables efficient electro-transfection while stimulating cells to produce high-quality EVs carrying functional RNAs. Article Title: Trans‐Coumaryl acetate mediates GRK5/NF‐κB/Nrf2 signaling axis to ameliorate septic acute kidney injury Article Snippet: Cell surfaces or tissue sections were permeabilized with drops of 0.3% Tritonx‐100 (X100, Sigma‐Aldrich) for 10 min 5% bovine serum albumin (BSA, V900933, Sigma‐Aldrich) was added for closure for 30 min. NF‐κB primary antibody (14‐6731‐81, 1:100, Invitrogen) was added dropwise on the cell surface, and F4/80 primary antibody (MA5‐16363, 1:100, Invitrogen) was added dropwise on the surface of the tissue sections, and incubated overnight at 4°C. .. The next day, the tissue sections were incubated with FITC‐labeled goat anti‐rabbit IgG (F‐2765, 1:100, Invitrogen) for 1 h at 37°C away from light, and then they were exposed to Article Title: Heterologous expression of AtLEC1 and AtLEC1-LIKE transcription factors redirects carbon flux toward lipid accumulation in diatom Article Snippet: .. The cell pellet was then treated with 150 μl of Article Title: Biodegradable Janus sonozyme with continuous reactive oxygen species regulation for treating infected critical-sized bone defects Article Snippet: .. The cells were covered with primary antibody of the appropriate genus and incubated at room temperature for 60 min, washed with 0.1% Phosphate Buffer Saline Tween (PBST), then Article Title: Heterologous expression of AtLEC1 and AtLEC1-LIKE transcription factors redirects carbon flux toward lipid accumulation in diatom. Article Snippet: .. The cell pellet 175 was then treated with 150 μl of Saline:Article Title: Biodegradable Janus sonozyme with continuous reactive oxygen species regulation for treating infected critical-sized bone defects. Article Snippet: .. The cells were covered with primary antibody of the appropriate genus and incubated at room temperature for 60min, washed with 0.1% Phosphate Buffer Saline Tween (PBST), then Article Title: Biodegradable Janus sonozyme with continuous reactive oxygen species regulation for treating infected critical-sized bone defects Article Snippet: .. The cells were covered with primary antibody of the appropriate genus and incubated at room temperature for 60 min, washed with 0.1% Phosphate Buffer Saline Tween (PBST), then Staining:Article Title: Biodegradable Janus sonozyme with continuous reactive oxygen species regulation for treating infected critical-sized bone defects. Article Snippet: .. The cells were covered with primary antibody of the appropriate genus and incubated at room temperature for 60min, washed with 0.1% Phosphate Buffer Saline Tween (PBST), then Article Title: Efficient, High-Quality Engineering of Therapeutic Extracellular Vesicles on an Integrated Nanoplatform. Article Snippet: Engineered extracellular vesicles (EVs) have been recognized as important therapeutics for gene and cell therapy.. To achieve clinically desired therapy, technologies for EV engineering have high demands on the efficacy in producing EVs and their qualities, which, however, remain challenging to conventional routes due to their limited control on therapeutic payload delivery, EV secretion, and extracellular microenvironments.. Here, we report a nanoplatform (denoted as PURE) that enables efficient electro-transfection while stimulating cells to produce high-quality EVs carrying functional RNAs. Article Title: Trans‐Coumaryl acetate mediates GRK5/NF‐κB/Nrf2 signaling axis to ameliorate septic acute kidney injury Article Snippet: Cell surfaces or tissue sections were permeabilized with drops of 0.3% Tritonx‐100 (X100, Sigma‐Aldrich) for 10 min 5% bovine serum albumin (BSA, V900933, Sigma‐Aldrich) was added for closure for 30 min. NF‐κB primary antibody (14‐6731‐81, 1:100, Invitrogen) was added dropwise on the cell surface, and F4/80 primary antibody (MA5‐16363, 1:100, Invitrogen) was added dropwise on the surface of the tissue sections, and incubated overnight at 4°C. .. The next day, the tissue sections were incubated with FITC‐labeled goat anti‐rabbit IgG (F‐2765, 1:100, Invitrogen) for 1 h at 37°C away from light, and then they were exposed to Article Title: Human umbilical cord mesenchymal stem cell -derived exosomes alleviated the toxic effects induced by lead on HK-2 cells via regulating the unfolded protein response. Article Snippet: .. Chemicals and reagents utilized in this study are listed below: Lead acetate (Pb(Ac)2, Aladdin, China), High-glucose dulbecco’s modified eagle medium (DMEM, Gibco, USA), Trypsin - EDTA (TE, Gibco, USA), Fetal bovine serum (FBS, Gibco, USA), Penicillin / streptomycin (Gibco, USA), Cell Counting Kit-8 (CCK-8, Abbkine, China), Trizol RNA isolation reagent (Invitrogen, USA), TransScript RT reagent kit (TransGen Biotech, China), SYBR Green qPCR Kit (Accurate Biology, China), Bestar® SybrGreen qPCR Mastermix (Accurate Biology, China), Article Title: Therapeutic potential of Platycodin D in allergic asthma through anti-inflammatory and anti-remodeling effects. Article Snippet: Background: Allergic asthma (AA) is a prevalent chronic respiratory disease characterized by airway hyperresponsiveness (AHR) and chronic inflammation, significantly impairing patients’ quality of life.. Purpose: This study investigates the therapeutic effects of Platycodin D (PLD) on AA and its underlying mechanisms via the EGFR/PI3K/Akt signaling pathway.. Methods: In vitro, BEAS-2B cells treated with IL-4 and IL-13 simulated asthma’s inflammatory environment. Article Title: Heterologous expression of AtLEC1 and AtLEC1-LIKE transcription factors redirects carbon flux toward lipid accumulation in diatom Article Snippet: .. The cell pellet was then treated with 150 μl of Article Title: Biodegradable Janus sonozyme with continuous reactive oxygen species regulation for treating infected critical-sized bone defects Article Snippet: .. The cells were covered with primary antibody of the appropriate genus and incubated at room temperature for 60 min, washed with 0.1% Phosphate Buffer Saline Tween (PBST), then Article Title: Heterologous expression of AtLEC1 and AtLEC1-LIKE transcription factors redirects carbon flux toward lipid accumulation in diatom. Article Snippet: .. The cell pellet 175 was then treated with 150 μl of Fluorescence:Article Title: Trans‐Coumaryl acetate mediates GRK5/NF‐κB/Nrf2 signaling axis to ameliorate septic acute kidney injury Article Snippet: Cell surfaces or tissue sections were permeabilized with drops of 0.3% Tritonx‐100 (X100, Sigma‐Aldrich) for 10 min 5% bovine serum albumin (BSA, V900933, Sigma‐Aldrich) was added for closure for 30 min. NF‐κB primary antibody (14‐6731‐81, 1:100, Invitrogen) was added dropwise on the cell surface, and F4/80 primary antibody (MA5‐16363, 1:100, Invitrogen) was added dropwise on the surface of the tissue sections, and incubated overnight at 4°C. .. The next day, the tissue sections were incubated with FITC‐labeled goat anti‐rabbit IgG (F‐2765, 1:100, Invitrogen) for 1 h at 37°C away from light, and then they were exposed to Article Title: Therapeutic potential of Platycodin D in allergic asthma through anti-inflammatory and anti-remodeling effects. Article Snippet: Background: Allergic asthma (AA) is a prevalent chronic respiratory disease characterized by airway hyperresponsiveness (AHR) and chronic inflammation, significantly impairing patients’ quality of life.. Purpose: This study investigates the therapeutic effects of Platycodin D (PLD) on AA and its underlying mechanisms via the EGFR/PI3K/Akt signaling pathway.. Methods: In vitro, BEAS-2B cells treated with IL-4 and IL-13 simulated asthma’s inflammatory environment. Microscopy:Article Title: Trans‐Coumaryl acetate mediates GRK5/NF‐κB/Nrf2 signaling axis to ameliorate septic acute kidney injury Article Snippet: Cell surfaces or tissue sections were permeabilized with drops of 0.3% Tritonx‐100 (X100, Sigma‐Aldrich) for 10 min 5% bovine serum albumin (BSA, V900933, Sigma‐Aldrich) was added for closure for 30 min. NF‐κB primary antibody (14‐6731‐81, 1:100, Invitrogen) was added dropwise on the cell surface, and F4/80 primary antibody (MA5‐16363, 1:100, Invitrogen) was added dropwise on the surface of the tissue sections, and incubated overnight at 4°C. .. The next day, the tissue sections were incubated with FITC‐labeled goat anti‐rabbit IgG (F‐2765, 1:100, Invitrogen) for 1 h at 37°C away from light, and then they were exposed to Modification:Article Title: Human umbilical cord mesenchymal stem cell -derived exosomes alleviated the toxic effects induced by lead on HK-2 cells via regulating the unfolded protein response. Article Snippet: .. Chemicals and reagents utilized in this study are listed below: Lead acetate (Pb(Ac)2, Aladdin, China), High-glucose dulbecco’s modified eagle medium (DMEM, Gibco, USA), Trypsin - EDTA (TE, Gibco, USA), Fetal bovine serum (FBS, Gibco, USA), Penicillin / streptomycin (Gibco, USA), Cell Counting Kit-8 (CCK-8, Abbkine, China), Trizol RNA isolation reagent (Invitrogen, USA), TransScript RT reagent kit (TransGen Biotech, China), SYBR Green qPCR Kit (Accurate Biology, China), Bestar® SybrGreen qPCR Mastermix (Accurate Biology, China), CCK-8 Assay:Article Title: Human umbilical cord mesenchymal stem cell -derived exosomes alleviated the toxic effects induced by lead on HK-2 cells via regulating the unfolded protein response. Article Snippet: .. Chemicals and reagents utilized in this study are listed below: Lead acetate (Pb(Ac)2, Aladdin, China), High-glucose dulbecco’s modified eagle medium (DMEM, Gibco, USA), Trypsin - EDTA (TE, Gibco, USA), Fetal bovine serum (FBS, Gibco, USA), Penicillin / streptomycin (Gibco, USA), Cell Counting Kit-8 (CCK-8, Abbkine, China), Trizol RNA isolation reagent (Invitrogen, USA), TransScript RT reagent kit (TransGen Biotech, China), SYBR Green qPCR Kit (Accurate Biology, China), Bestar® SybrGreen qPCR Mastermix (Accurate Biology, China), Isolation:Article Title: Human umbilical cord mesenchymal stem cell -derived exosomes alleviated the toxic effects induced by lead on HK-2 cells via regulating the unfolded protein response. Article Snippet: .. Chemicals and reagents utilized in this study are listed below: Lead acetate (Pb(Ac)2, Aladdin, China), High-glucose dulbecco’s modified eagle medium (DMEM, Gibco, USA), Trypsin - EDTA (TE, Gibco, USA), Fetal bovine serum (FBS, Gibco, USA), Penicillin / streptomycin (Gibco, USA), Cell Counting Kit-8 (CCK-8, Abbkine, China), Trizol RNA isolation reagent (Invitrogen, USA), TransScript RT reagent kit (TransGen Biotech, China), SYBR Green qPCR Kit (Accurate Biology, China), Bestar® SybrGreen qPCR Mastermix (Accurate Biology, China), SYBR Green Assay:Article Title: Human umbilical cord mesenchymal stem cell -derived exosomes alleviated the toxic effects induced by lead on HK-2 cells via regulating the unfolded protein response. Article Snippet: .. Chemicals and reagents utilized in this study are listed below: Lead acetate (Pb(Ac)2, Aladdin, China), High-glucose dulbecco’s modified eagle medium (DMEM, Gibco, USA), Trypsin - EDTA (TE, Gibco, USA), Fetal bovine serum (FBS, Gibco, USA), Penicillin / streptomycin (Gibco, USA), Cell Counting Kit-8 (CCK-8, Abbkine, China), Trizol RNA isolation reagent (Invitrogen, USA), TransScript RT reagent kit (TransGen Biotech, China), SYBR Green qPCR Kit (Accurate Biology, China), Bestar® SybrGreen qPCR Mastermix (Accurate Biology, China), Real-time Polymerase Chain Reaction:Article Title: Human umbilical cord mesenchymal stem cell -derived exosomes alleviated the toxic effects induced by lead on HK-2 cells via regulating the unfolded protein response. Article Snippet: .. Chemicals and reagents utilized in this study are listed below: Lead acetate (Pb(Ac)2, Aladdin, China), High-glucose dulbecco’s modified eagle medium (DMEM, Gibco, USA), Trypsin - EDTA (TE, Gibco, USA), Fetal bovine serum (FBS, Gibco, USA), Penicillin / streptomycin (Gibco, USA), Cell Counting Kit-8 (CCK-8, Abbkine, China), Trizol RNA isolation reagent (Invitrogen, USA), TransScript RT reagent kit (TransGen Biotech, China), SYBR Green qPCR Kit (Accurate Biology, China), Bestar® SybrGreen qPCR Mastermix (Accurate Biology, China), Acid Assay:Article Title: Human umbilical cord mesenchymal stem cell -derived exosomes alleviated the toxic effects induced by lead on HK-2 cells via regulating the unfolded protein response. Article Snippet: .. Chemicals and reagents utilized in this study are listed below: Lead acetate (Pb(Ac)2, Aladdin, China), High-glucose dulbecco’s modified eagle medium (DMEM, Gibco, USA), Trypsin - EDTA (TE, Gibco, USA), Fetal bovine serum (FBS, Gibco, USA), Penicillin / streptomycin (Gibco, USA), Cell Counting Kit-8 (CCK-8, Abbkine, China), Trizol RNA isolation reagent (Invitrogen, USA), TransScript RT reagent kit (TransGen Biotech, China), SYBR Green qPCR Kit (Accurate Biology, China), Bestar® SybrGreen qPCR Mastermix (Accurate Biology, China), Membrane:Article Title: Human umbilical cord mesenchymal stem cell -derived exosomes alleviated the toxic effects induced by lead on HK-2 cells via regulating the unfolded protein response. Article Snippet: .. Chemicals and reagents utilized in this study are listed below: Lead acetate (Pb(Ac)2, Aladdin, China), High-glucose dulbecco’s modified eagle medium (DMEM, Gibco, USA), Trypsin - EDTA (TE, Gibco, USA), Fetal bovine serum (FBS, Gibco, USA), Penicillin / streptomycin (Gibco, USA), Cell Counting Kit-8 (CCK-8, Abbkine, China), Trizol RNA isolation reagent (Invitrogen, USA), TransScript RT reagent kit (TransGen Biotech, China), SYBR Green qPCR Kit (Accurate Biology, China), Bestar® SybrGreen qPCR Mastermix (Accurate Biology, China), Data-independent acquisition:Article Title: Human umbilical cord mesenchymal stem cell -derived exosomes alleviated the toxic effects induced by lead on HK-2 cells via regulating the unfolded protein response. Article Snippet: .. Chemicals and reagents utilized in this study are listed below: Lead acetate (Pb(Ac)2, Aladdin, China), High-glucose dulbecco’s modified eagle medium (DMEM, Gibco, USA), Trypsin - EDTA (TE, Gibco, USA), Fetal bovine serum (FBS, Gibco, USA), Penicillin / streptomycin (Gibco, USA), Cell Counting Kit-8 (CCK-8, Abbkine, China), Trizol RNA isolation reagent (Invitrogen, USA), TransScript RT reagent kit (TransGen Biotech, China), SYBR Green qPCR Kit (Accurate Biology, China), Bestar® SybrGreen qPCR Mastermix (Accurate Biology, China), Glutathione Assay:Article Title: Human umbilical cord mesenchymal stem cell -derived exosomes alleviated the toxic effects induced by lead on HK-2 cells via regulating the unfolded protein response. Article Snippet: .. Chemicals and reagents utilized in this study are listed below: Lead acetate (Pb(Ac)2, Aladdin, China), High-glucose dulbecco’s modified eagle medium (DMEM, Gibco, USA), Trypsin - EDTA (TE, Gibco, USA), Fetal bovine serum (FBS, Gibco, USA), Penicillin / streptomycin (Gibco, USA), Cell Counting Kit-8 (CCK-8, Abbkine, China), Trizol RNA isolation reagent (Invitrogen, USA), TransScript RT reagent kit (TransGen Biotech, China), SYBR Green qPCR Kit (Accurate Biology, China), Bestar® SybrGreen qPCR Mastermix (Accurate Biology, China), |
